Cutaneous Lymphocyte-assiciated Antigen (CLA) is a 140kD homodimer protein recognized by a unique monoclonal antibody.
It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells.
CLA is a carbohydrate epitope of sialic acid- and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes.
CLA is a subset ligand for E-selectin, P-selectin, and L-selectin.
It plays a role in memory lymphocyte homing, tethering and rolling.
Treatment of activated HUVEC cells with this antibody inhibits lymphocyte adhesion.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 5ul/10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：rat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human Cutaneous Lymphocyte Antigen. Species Crossreactivity: mouse
