Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor.
The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action.
Applications:Suitable for use in Immunoprecipitation, Immunohistochemistry, Inhibition and as a weak IGF-like Agonist in Bioassays.
Other applications not tested.
Recommended Dilutions:Immunoprecipitation: 2ug/mg protein lysate.
Use Protein G
Immunohistochemistry (Frozen and formalin/paraffin): 2-4ug/ml for 30 min.
at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min.
followed by cooling at RT for 20 minutes.
Please note that EDTA is better than citrate for epitope unmasking
Bioassays: Thymidine incorporation into DNAOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from ascites.
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes the alpha-subunit of human type I Insulin-like Growth Factor Receptor (IGF-1R). Shows no cross-reaction with insulin receptor. Weakly reacts with rabbit.
Clone # USB：24-31
Isotype：IgG1
Calc Applications Abbrev：IHC IP
Calc Crossreactivity：Hu
Immunogen：IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA. Epitope: aa283-440 (exon 4-6).
