Dopamine is a neurotransmitter that plays important roles in the brain, particularly in dopamine pathways that control the motivational component of reward-motivated behavior.
These behavioral outputs are generated by the basal ganglia via its interaction with multiple brain areas that modulate sensorimotor, emotional, and cognitive information (1).
The brain’s major dopaminergic input is into the striatum, a region of the basal ganglia composed of GABAergic medium spiny neurons (MSNs).
Two major subpopulations of MSN exist in the striatum that are distinguished by the expression of dopamine receptor subtypes, the dopamine D(1) receptor subtype and the dopamine D(2) receptor subtype (D1R and D2R, respectively) (2,3).
As a family of proteins, dopamine receptors are a class of G protein-coupled receptors (GPCRs) consisting of 5 subtypes that, upon initiation, drive downstream signaling cascades that modulate neuronal function (1).
Dopamine receptors form homo- and hetero-multimers with subunits within their protein family but also with other GPCRs, including Adenosine Receptor A2a, suggesting that dopamine receptor activity might be finely tuned and altered under certain conditions (4).
Dopamine receptors have been studied as a therapeutic target for several neuropsychiatric and developmental disorders, as well as neurodegenerative diseases, including Parkinson’s disease (5-8).
Dopamine receptors are also expressed outside of the brain and may have diverse functions beyond the central nervous system, including regulating innate and adaptive immunity (9).
Applications:Suitable for use in Western Blot and Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Immunofluorescence (Frozen): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, 50% glycerol.
Specificity：Recognizes transfected levels of dopamine D(1) receptor/D1R by western blot and endogenous levels of total dopamine D(1) receptor/D1R protein in mouse brain tissue by immunofluorescence.
Clone # USB：E3H3S
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to residues surrounding Val438 of mouse dopamine D(1) receptor/D1R. Species Sequence Homology (100%): rat
