Human protease-activated receptor 2 (PAR2) is one of 4 members in a unique subclass of the Class A G protein- coupled receptor superfamily.
Receptor activation involves the proteolytic uncovering of a cryptic N-terminus receptor sequence that forms a tethered ligand and binds to the receptors within the extracellular loop 2.
This activates the receptors to initiate multiple signaling cascades.
In addition, short peptides (5-6 amino acids) synthesized based on the proteolytically revealed motif can activate the receptors without the cleavage by enzymes.
PAR2 is activated by trypsin and trypsin-like enzymes, and the cleavage site has been identified at residue Arg 36.
PAR2 plays an important role in the regulation of cardiovascular, gastrointestinal, and skin function.
PAR2 is expressed in human vascular tissues, pancreas, kidney, colon, liver, small intestine, and some tumor cell lines (1, 2).
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 1x10e6 cells using HT-29 human colon adenocarcinoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatgraphy from cell culture supernatant.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.
Clone # USB：7H40
Isotype：IgG2a
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：NS0 mouse myeloma cell line transfected with human PAR2
