Nectin-4 is a type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins.
It is both a homophilic and heterophilic (with Nectin-1) cell adhesion molecule that is expressed in the embryo and in breast carcinoma.
A soluble form of Nectin-4 is generated from the membrane protein via the action of TACE/ADAM-17.
The extracellular domain of human Nectin-4 shares 90% and 92% amino acid sequence homology with the corresponding regions of mouse and rat Nectin-4, respectively.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 10e6 MCF-7 human breast cancer cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human Nectin-4. Does not crossreact with recombinant human Nectin-1, -2, -3 or recombinant mouse Nectin-4.
Clone # USB：7H96
Isotype：IgG2b
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly27-Val351 of human Nectin 4 expressed in NS0 cells. Species Sequence Homology: mouse; 90%, rat; 92%.
