Eck (EphA2) is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1-A5.
A and B class Eph proteins have a common structural organization.
The human EphA2 cDNA encodes a 976aa precursor including a 24aa signal sequence, a 510aa extra- cellular domain (ECD), a 24aa transmembrane segment and a 418aa cytoplasmic domain.
The ECD contains an N-terminal globular domain, a cysteine-rich domain and two fibronectin type III domains.
The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM).
The ECD of human EphA2 shares 90-94% aa sequence identity with mouse, bovine, and canine EphA2 and ~45% aa sequence identity with human EphA1, 3, 4, 5, 7 and 8.
EphA2 becomes autophosphorylated following ligand binding and then interacts with SH2 domain- containing PI3-kinase to activate MAPK pathways.
Reverse signaling is also propagated through the Ephrin ligand.
Transcription of EphA2 is dependent on the expression of E-Cadherin and can be induced by p53 family transcription factors.
EphA2 is upregulated in breast, prostate and colon cancer vascular endothelium.
Its ligand, EphrinA1, is expressed by the local tumor cells.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 10ul labels 10e6 A431 human epithelial carcinoma cell line
Optimal dilutions to be determined by the researcher.
Excitation:620-650nm
Emission:660-670nm
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human Eck (EphA2). Does not crossreact with recombinant mouse EphA4, -A5, -A6, -A7, -A8 or recombinant rat Eph-B1.
Clone # USB：7H150
Isotype：IgG2a
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln25-Asn534 from human Eck expressed in NS0 cells.
