Studying the monoaminergic system has been of great interest and importance since dopamine, noradrenaline and adrenaline are the neurotransmitters involved in multiple physiological and pathophysiological conditions.
DBH is the enzyme responsible for the biosynthetic hydroxylation of dopamine to noradrenaline.
Applications:Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:30-1:50Immunocytochemistry: 1:30-1:50Optimal dilutions to be determined by the researcher.r.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mL
抗体種類：Mab
宿主：mouse
Source Antibody：rat
Grade：Supernatant
Purity：Tissue culture supernatant
Form：Supplied as a lyophilized powder. No preservative added.. Reconstitute with 2mL ddH2O.
Specificity：Recognizes rat DBH. Specifically stains DBH-containing neurons and fibers in the rat central nervous system. Does not recognize mouse, human, rabbit, guinea pig, feline, bovine. Specifically recognizes two bands of molecular weight 70 and 75kD, corresponding to the soluble and membrane bound forms of the enzyme, respectively. Due to the high sensitivity of the DBH antibody, the presence of the enzyme can be immunologically detected even in areas where only scattered DBH-containing fibers are present.
