MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor.
MDM2 antagonizes the transcriptional activity of p53 in two main ways.
The N-terminal of MDM2 interacts and disables the transactivation domain of p53.
In addition, the C-terminal E3 ligase region of MDM2 can attach monoubiquitin to p53 resulting in the transport of p53 from the nucleus to the cytoplasm, thereby preventing the interaction of p53 with target DNA sequences.
In addition, MDM2 may also attach polyubiquitin chains to p53, resulting in the degradation of p53 by the proteosome.
MDM2 may produce these effects in cooperation with the Mdmx protein.
Overall, MDM2 inhibits the ability of p53 to induce cell cycle arrest or apoptosis, and may contribute to oncogenesis.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5ug/ml detected MDM2 on 10ug of A-549 cell lysate
Optimal dilutions to be determined by the researcher.
Control:A-549 cell lysate
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from culture supernatant.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
