Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) is a tyrosine kinase substrate which has a cysteine-rich domain which resembles the SPR domain at the carboxy terminus.
SPRED1 associated with Ras blocks phosphorylation of Raf and disrupts MAP kinase signaling during the differentiation of neuronal cells and myocytes.
A mutation in SPRED1 has been linked to Lugius syndrome, an autosomal dominant RAS-MAPK pathway syndrome.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml in 10ug of mouse brain tissue lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes Spred-1. Species Crossreactivity: mouse and rat
Clone # USB：M23-P2G3
Isotype：IgG1
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Mo Rt
Immunogen：Linear peptide corresponding to human Spred-1. Species Sequence Homology: human (100%)
