Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
The T7-tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10.
The T7-tag has been used extensively as a general epitope tag in many expression vectors, including the pET system that is based on T7 RNA polymerase expression systems (1).
Applications:Suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000; incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight
Immunofluorescence (IC): 1:1600-1:3200 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Ascites
Purity：Ascites.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes recombinant proteins containing the T7 epitope tag. The antibody recognizes the T7-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. 046308 detects exogenously expressed T7-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may detect several non-specific bands of unknown origin in rat cell lines.
