Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
The ALFA-tag is a highly versatile epitope tag made from a hydrophilic, 15 amino acid sequence with a high propensity to form a small, stable α-helix structure (1).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000; incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Ascites
Purity：Ascites.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/mL BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes exogenously expressed proteins containing the ALFA epitope tag. The antibody recognizes the ALFA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. 046306 detects exogenously expressed ALFA-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may detect several non-specific bands of unknown origin in rat cell lines. Species Crossreactivity: all
Clone # USB：E1R1A
Isotype：IgG1,k
Calc Applications Abbrev：WB
Immunogen：Synthetic peptide corresponding to residues of the ALFA epitope tag.
