Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance.
It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells.
It also induces regulatory T cells and anti-inflammatory macrophages.
HLA-G is important e.g.
for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other.
On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance.
HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome.
Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule.
It concerns both transmembrane and soluble HLA-G isoforms.
Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response.
For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization.
Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia.
Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance.
On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells.
Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect.
Monitoring of HLA-G in patients thus has a wide usage.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1-4ug/ml; intracellular stainingOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allows to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Clone # USB：5A6G7
Isotype：IgG1
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
