HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin.
They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defence.
Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g.
HLA-E, HLA-G.
Applications:Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1-4ug/mlImmunohistochemistry: frozenOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes an extracellular epitope on human HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.
Clone # USB：TP25.99SF
Isotype：IgG1
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Hu
Immunogen：IFN-gamma-treated human melanoma cells Colo 38
