83-5977-42 HLA Class 1 Antigen G (HLA Cass I Histocompatibility Antigen alpha Chain G, HLA G Antigen, HLA G, HLAG, HLA-G, HLA-6.0, Major Histocompatibility Complex Class I G, MHC-G) (APC) 100ug 046287
83-5977-42 HLA Class 1 Antigen G (HLA Cass I Histocompatibility Antigen alpha Chain G, HLA G Antigen, HLA G, HLAG, HLA-G, HLA-6.0, Major Histocompatibility Complex Class I G, MHC-G) (APC) 100ug 046287
HLA-G belongs to the HLA class I heavy chain paralogues.
This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin).
The heavy chain is anchored in the membrane.
HLA-G is expressed on fetal derived placental cells.
The heavy chain is approximately 45kD and its gene contains 8 exons.
Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1-5ug/ml using JEG-3 human choriocarcinoma cell line
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size:100ug
抗体種類:Mab
宿主:mouse
Source Antibody:human
Grade:Purified
Purity:Purified
Form:Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity:Recognizes the native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form.
Clone # USB:MEM-G/9
Isotype:IgG1
Calc Applications Abbrev:FC
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human HLA-G refolded with beta2-microglobulin and peptide.