Immunoglobulin E (IgE) is a 180kD soluble protein serving as an antigen-specific unit of mast cell effector mechanisms.
IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically.
Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor.
Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human IgE. Epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
