AHNAK1 (Desmoyokin) is a large (700kD) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels.
AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation.
In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture.
In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.
Applications:Suitable for use in Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry permeabilization is required
Immunohistochemistry: frozenOptimal dilutions to be determined by the researcher.
Positive Control:HeLa human cervix carcinoma cell line
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide
Specificity：Recognizes human AHNAK1. Species Crossreactivity: mouse
Clone # USB：EM-09
Isotype：IgG1
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Bacterially expressed fragment of N-terminal domain of human AHNAK1.
