TIAR is an ubiquitously expressed RNA-binding protein, which regulates translational control, splicing, and other activities, including apoptosis.
TIAR attenuates CDK1 activity, and is essential for the G2/M checkpoint.
It accumulates in nuclear foci in late G2 phase and prophase in cells under replication stress.
In steady state TIAR shuttles between the cytoplasm and the nucleus, probably as a part of nucleocytoplasmic transport of mRNA, but under stress conditions it accumulates mRNA molecules in granules and prevents their translation.
Nucleolytic activity of TIAR against attacked target cells of cytotoxic lymphocytes has also been reported.
Similarly, e.g.
in permeabilized thymocytes TIAR triggers DNA fragmentation.
Applications:Suitable for use in Western Blot, ELISA, Flow Cytometry, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 5ug/ml using intracellular stainingImmunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes human TIAR. Species Crossreactivity: mouse and primate
Clone # USB：6E3
Isotype：IgG2a,k
Calc Applications Abbrev：E FC IC IHC WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：GST-tagged recombinant protein corresponding to human TIAR
