DSP (desmoplakin) is the major high molecular weight protein of desmosomes, the most common type of intercellular junction in vertebrate epithelial cells.
DSP is involved in the organization of the desmosomal cadherin-plakoglobin complex, as well as in the anchoring of intermediate filaments to desmosomes.
These functions are thought to impart tensile strength and integrity to epithelial and vascular tissues.
Mutations in DSP result in several clinical phenotypes, ranging from mild skin conditions, to severe skin blistering with subsequent dehydration, to lethal heart defects.
Two major desmoplakin isoforms, DSPI and DSPII, are encoded by alternative mRNA transcripts differentially spliced from the same gene.
Human DSPI is 2,871 amino acids (aa) in length, while DSPII lacks part of a central alpha-helical rod domain, and is 599 aa shorter.
Applications:Suitable for use in Western Blot.
Other applications have not been tested
Recommended Dilutions:Western Blot: 0.5ug/ml in COLO 205 human colorectal adenocarcinoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human Desmoplakin
Clone # USB：23K13
Isotype：IgG1
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asn1796-Val2046 from human Desmoplakin, expressed in E. coli.
