Nucleoprotein (UniProt: R9UM87; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 14254601) in Middle East respiratory syndrome-related coronavirus (MERS-CoV).
The MERS-CoV is a positive-strand RNA virus of the betacoronavirus genus within the Coronaviridae family that causes severe respiratory syndrome in human.
The mature MERS-CoV contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S).
Nucleoprotein of MERS-CoV is a homooligomeric multifunctional protein that plays roles in genome encapsidation, RNA synthesis during replication, host translational shutoff, and innate immunity disruption.
It packages the positive strand viral genome RNA into helical ribonucleocapsid and is involved in virion assembly through its interactions with the viral genome and membrane protein M.
The nucleoprotein is reportedly organized into two folded domains known as the N-terminal domain (NTD) and the C-terminal domain (CTD) that are separated by a disordered region (LKR) containing a serine/arginine stretch.
046250 displays specificity for MERS-CoV nucleoprotein and does not show cross-reactivity with nucleoprotein from other related viruses, including SARS coronavirus.
(Ref.: Nguyen, THV., et al.
(2019).
Acta Crystallogr.
D.
Struct.
Biol.
75(1); 8-15; Yamaoka, Y., et al.
(2016).
Front.
Microbiol.
7; Article 509).
Applications:Suitable for use in Western Blot, Immunoprecipitation and ELISA.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes the nucleoprotein from Middle East respiratory syndrome-related coronavirus (MERS-CoV). Does not react with nucleoproteins from other related viruses, including SARS coronavirus
Clone # USB：46
Isotype：IgG1,k
Calc Applications Abbrev：E IP WB
Immunogen：His-tagged recombinant protein fragment corresponding to 292 amino acids from the nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV).
