83-5976-90　Rabies Virus, Strain ERA 100ug　046248

特徴

• Glycoprotein (UniProt: P03524) of Rabies virus of Evelyn-Rokitnicki-Abelseth (ERA) strain is encoded by the G gene.
• Rabies virus belongs to the Lyssavirus serogroup of viruses.
• The glycoprotein of Rabies virus is a homotrimer, single-pass type I membrane protein that attaches the virus to host cellular receptor and thereby inducing endocytosis of the virion.
• In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane.
• It has been shown that the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and facilitate Rabies virus entry into cells.
• Arginine 352 is considered to be highly involved in Rabies virus pathogenicity and a mutation at this site dramatically attenuates the virus.
• Rabies virus glycoprotein is synthesized with a signal peptide (aa 1-19) that is subsequently cleaved off.
• It has two topological domains (aa 20-459 virion surface; 481-524 intravirion) and a helical domain (aa 460-480).
• Glycosylation of viral glycoprotein is considered to be crucial for glycoprotein export at the cell surface.
• 046248 is shown to recognize most Rabies virus strains, however it does not display neutralizing activity.
• (Ref.: Schumacher, CL et al.
• (1989).
• J.
• Clin.
• Invest.
• 84(3); 971-975; Bunschoten, H et al.
• (1989).
• J.
• Gen.
• Virol.
• 70(2); 291-298).
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.