Glycoprotein of Evelyn Rokitniki Abelseth (ERA) SAD derived Rabies virus strain (UniProt: P03524) is encoded by the G gene.
It belongs to the lyssavirus glycoprotein of the Rhabdoviridae family.
This glycoprotein is a single pass type I virion membrane protein that helps in attaching the virus to host cellular receptor, inducing endocytosis of the virion.
In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane.
It has been shown that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (N-CAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and facilitate rabies virus entry into cells.
Arg352 of Glycoprotein is highly involved in rabies virus pathogenicity and mutation at this site dramatically attenuates the virus.
Applications:Suitable for use in Flow Cytometry and Neutralization.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1ug detected Rabies virus in 10e6 L929 cells expressing rabies protein
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes a glycoprotein of the ERA strain of rabies virus in L929 cells that express rabies protein.
Clone # USB：1112-1
Isotype：IgG1,k
Calc Applications Abbrev：FC N
Immunogen：Glycoprotein of ERA (Evelyn Rokitniki Abelseth) strain Rabies virus.
