HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor.
Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin.
Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined.
In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).
Applications:Suitable for use in ELISA, Immunoprecipitation and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1-4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes native extracellular part of human HLA-E, an ubiquitously expressed non-classical MHC class I molecule, as well as free HLA-E.
Clone # USB：3D12
Isotype：IgG1
Calc Applications Abbrev：E FC IP
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human HLA-E.
