NG2 / chondroitin sulfate proteoglycan 4 is expressed on glial cell populations, but not on normal hepatopoietic cells.
It is an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells, where it plays role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes, and supports signaling pathways important for tumor invasion and growth.
NG2 also serves as an AML blast tumor marker associated with poor prognosis.
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1-4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes human NG2 at ~220-300kD.
Clone # USB：7.1
Isotype：IgG1
Calc Applications Abbrev：FC IC IP WB
Calc Crossreactivity：Hu
Immunogen：Human bone marrow stromal cells infected with SV-40
