83-5976-59　CD79a (CD 79a, BCR alpha, Ig-alpha, MB-1, IGA, B Cell Antigen Receptor Complex Associated Protein alpha Chain precursor, CD79A Antigen) (APC) 100Tests　046232

特徴

• CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta).
• They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR).
• CD79a and b are the first components of BCR that are expressed developmentally.
• They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin.
• Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins.
• At the plasma cell stage, CD79a is present as an intracellular component.
• CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry (intracellular): 10ul labels 10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4℃ in the dark.
• DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.