GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism.
Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate.
During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.
It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Applications:Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry: 10ug/ml; membrane permeabilization (acetone) is required
Flow Cytometry: 3-12ug/ml.
Intraacrosomal staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in TBS, pH 8.0, 15mM sodium azide
Specificity：Recognizes human GAPDS. Species Crossreactivity: porcine
Clone # USB：Hs-8
Isotype：IgM
Calc Applications Abbrev：FC IC WB
Calc Crossreactivity：Hu Po
Immunogen：Freshly ejaculated human sperm were washed in PBS and extracted in 3% acetic acid, 10% glycerol and 30mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid
