GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is an intracellular homotetramerizing enzyme, which catalyzes oxidation of glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate during glycolysis, and has also functions in DNA replication, gene expression, and microtubule bundling.
As it binds to many proteins, its functions are very broad.
GAPDH plays also roles in cancer progression, apoptosis, Huntington disease and Alzheimer´s disease.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Frozen sectionsWestern Blot: 1ug/ml; both reducing and non-reducing conditions;Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) at ~37kD. Does not react with mouse or porcine
