BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine.
Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry (Immunofluorescent intracellular staining): 5ul labels 10e6 cells in 100ulOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size:100Tests
抗体種類:Mab
宿主:mouse
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).