83-5976-41　Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen) (PE) 100Tests　046219-PE

特徴

• Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345kD and 395kD), both of which are identified by the antibody clone Ki-67.
• The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells.
• It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes.
• Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells of 100ul whole blood or 1x10e6 cells in suspensionOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4℃ in the dark.
• DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.