Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345kD and 395kD), both of which are identified by the antibody clone Ki-67.
The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells.
It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes.
Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Applications:Suitable for use in Flow Cytometry, Immunocytochemistry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1-5ug/mlImmunocytochemistry 1ug/ml using paraformaldehyde fixationOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity：Recognizes human Ki67. Species Crossreactivity: bovine
Clone # USB：Ki-67
Isotype：IgG1
Calc Applications Abbrev：FC IC IHC WB
Calc Crossreactivity：Bo Hu
Immunogen：Nuclei of the Hodgkin lymphoma cell line L428
