SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development.
Binding to Grb2 plays a pivotal role in signal transduction.
Hubener et al.
(2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA.
The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1-5ug/ml with intracellular staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human SHP2-interacting transmembrane adapter protein (SIT). Weaker reactivity was observed with mouse homolog.
Clone # USB：SIT-01
Isotype：IgG1
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Bacterially produced recombinant protein corresponding to human SIT, intracellular fragment.
