83-5976-22　Cas12j (CasΦ) 100ul　046203

特徴

• There has been much recent interest in gene editing and other genetic manipulations by CRISPR-Cas family enzymes.
• Recent ecosystem and microbiome DNA sequencing and assembly characterized genomes of “huge phages” or megaphages, see Al-Shayeb et al.
• Nature 578:425-431 (2020) and showed that they contained novel Cas family enzymes.
• The lab of Nobel prize winner Jennifer Doudna characterized these Cas enzymes and showed that they could be used to perform CRISPR, but had the significant advantage that they were much smaller in molecular size than other Cas family enzymes such as the widely used Cas9 enzymes from S.
• pyogenes and S.
• aureus, see Pausch, P.
• et al.
• Science 369:333-337 (2020).
• One of these enzymes is referred to as CasΦ and also known as Cas-12j and was the focus of the Pausch et al.
• paper.
• The use of such smaller Cas family enzymes leaves more room for other nucleic acid sequences in AAV and other viral vectors which typically have a limited DNA capacity, thus allowing more versatility for new CRISPR based manipulations.
• Applications:Suitable for use in Western Blot and Immunofluorescence/Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence (IC): 1:1000Western Blot: 1:1000-1:2000 with crude homogenates of HEK293 cells transfected with the Cas-phi-2 DNA,Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.