Parvalbumin is a low molecular weight cytoplasmic Calcium binding protein containing the “EF hand” Calcium binding motif and is the first protein characterized in this subclass (1).
Parvalbumin is expressed in fast-contracting muscles, in the brain and in some endocrine tissues (2,3).
In brain it is particularly concentrated in Purkinje cells and interneurons in the molecular layer, but is also found in many cortical GABAergic interneurons.
These GABAergic interneurons in most cases express only one of three Calcium binding proteins, namely parvalbumin, calretinin or calbindin (4-6).
Each type of interneuron has distinct electrophysiological properties and as a result, different types of interneuron can be identified and classified based on their content of these three proteins (7).
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/ Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:5000Immunohistochemistry (PFA fixed) : 1:1000-1:5000Immunofluorescence (IC): 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Purified antibody in PBS, 5mM sodium azide, 50% glycerol.
Specificity：Recognizes human Parvalbumin. Species Crossreactivity: mouse, rat, bovine, porcine and equine. Does not cross-react with either calbindin or calretinin despite their related amino acid sequences.
Clone # USB：3C9
Isotype：IgG1
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Bo Eq Hu Mo Po Rt
Immunogen：Full-length recombinant protein corresponding to human Parvalbumin, expressed in E. coli.
