TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells.
It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein.
TNF-alpha easily forms stable trimers, but also other multimeric complexes.
In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 10ul labels 10e6 cell in 100ul.
Intracellular staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human tumor necrosis factor-alpha. Species Crossreactivity: porcine and primate
Clone # USB：MAb11
Isotype：IgG1,k
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu Mk Po
Immunogen：Recombinant protein corresponding to human Tumor Necrosis Factor a.
