TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells.
It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein.
TNF-alpha easily forms stable trimers, but also other multimeric complexes.
In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 4ul labels 10e6 cell in 100ul.
Intracellular staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
Aliquots are stable at -20℃ for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100Tests
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human tumor necrosis factor-alpha. Species Crossreactivity: porcine and primate
Clone # USB：MAb11
Isotype：IgG1,k
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu Mk Po
Immunogen：Recombinant protein corresponding to human Tumor Necrosis Factor a.
