83-5976-06　TNF alpha (Tumor Necrosis Factor alpha, TNFA, DIF, TNLG1F) (Biotin) 100ug　046194-Biotin

特徴

• TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells.
• It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein.
• TNF-alpha easily forms stable trimers, but also other multimeric complexes.
• In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
• Applications:Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: paraformaldehyde-fixed, saponin-treated frozen tissue sections
• Flow Cytometry: Intracellular staining
• ELISA (detection): in combination with capture antibody 046195Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4℃ if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20℃.
• Aliquots are stable at -20℃ for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.