Complement component 3 (C3) is a 185kD glycoprotein composed of two chains linked by a disulfide bond.
It is the fourth complement component to react in the classical pathway and is a key protein in the alternative and the lectin complement activation pathways.
C3b is proteolytically generated from C3 by cleavage of the C3a-C3b peptide bond in the protein by C3 convertase.
C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Macrophages and neutrophils recognize C3b by the complement receptor 1 (CR1, CD35).
Opsonization of target surfaces with C3b is central to all three pathways of complement activation.
The proteolytically inactive forms of C3b, iC3b, are generated by cleavage of the alpha chain at one or two positions by factor I in the presence of co-factors, such as factor H.
A ~2 kD fragment of C3b, C3f, is generated when C3b is cleaved at two positions by factor I.
Although iC3b is less active than C3b, iC3b’s interactions with lymphoid and phagocytic cells via CR2 (CD21) and CR3 (CD11b/CD18) can expand target-specific B and T cells.
iC3b, can be cleaved to form C3c and C3dg.
Further proteolytic cleavage of C3dg generates C3d and C3g.
Applications:Suitable for use in RIA, ELISA, Flow Cytometry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/mlFlow Cytometry: 1-2ug/10e6 cells in 100ulELISA (direct): 0.01-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human C3, C3b and iC3b. Does not cross-react with C3d.
