83-5976-01　Plasma Kallikrein (KLKB1) 100ug　046189

特徴

• Human Plasma Kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma by binding to high molecular weight (HMW) kininogen or as a free zymogen.
• Once activated by its physiological activator, factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine.
• It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin.
• It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase.
• Thus, it plays an important role in blood pressure regulation, fibrinolysis, and neutrophil activation (1).
• Human Plasma Kallikrein precursor contains a signal peptide (residues 1 to 19) and a pro form sequence (residues 20 to 638).
• Upon activation, the pro form is converted to a heavy chain and a light chain, which is linked by disulfide bonds and the latter contains the catalytic domain (2).
• The human Plasma Kallikrein pro form was expressed in the NS0 cells with a foreign signal peptide.
• The purified enzyme is mainly the pro form
• Applications:Suitable for use in Immunohistochemistry.
• Other applications have not been tested
• Recommended Dilutions:Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin-embedded sections of human liver Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.