83-5975-99　Kininogen 500ug　046188

特徴

• Kininogen, also known as alpha 2-thiol proteinase inhibitor, is a multi-function protein.
• There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms (1).
• The HMW form is synthesized as a 644 amino acid (aa) precursor with a signal peptide (aa 1‑18).
• The mature chain (aa 19‑644) is further processed into the heavy (aa 19‑380) and the light (aa 390‑644) chains.
• The active peptide bradykinin (aa 381‑389) is released, which has a variety of functions including muscle contraction, hypotension and inflammation.
• The heavy chain consists of three cystatin-like domains, which are responsible for inhibiting cysteine proteases.
• The light chain consists of a His-rich domain, which is associated with the clotting activity.
• In comparison to the HMW form, the LMW Kininogen (427 aa) has the same sequence in its heavy chain and bradykinin, but a different sequence in its light chain (aa 402‑427).
• The LMW form is not involved in blood clotting.
• Applications:Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot.
• Other applications have not been tested
• Recommended Dilutions:Western Blot: 1ug/ml with recombinant human Kininogen high molecular weight (HKa) proteinImmunohistochemistry: 0.5-25ug/ml in immersion fixed paraffin-embedded sections of human brain (substantia nigra)Immunoprecipitation: 25ug/ml with conditioned cell culture medium spiked with Recombinant Human Kininogen High Molecular Weight (HKa) Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.