Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an ~95-110kD type II transmembrane glycoprotein expressed in various tissues.
In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate.
As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g.
Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target.
In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract.
GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumors and is a target enzyme for diagnosis and treatment of prostate cancer.
Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4℃ in the dark.
DO NOT FREEZE! Stable at 4℃ for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes the extracellular domain (preferentially in native form) of human glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA.
