Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an ~95-110kD type II transmembrane glycoprotein expressed in various tissues.
In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate.
As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g.
Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target.
In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract.
GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumors and is a target enzyme for diagnosis and treatment of prostate cancer.
Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer, pH 6.0 is recommended
Western Blot: 1ug/ml, Resuspend approx.
50 mil.
cells in 1ml cold lysis buffer (1% NP-40).
Incubate 30 min on ice.
Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
Both reducing and non-reducing conditions
Optimal dilutions to be determined by the researcher.
Western Blot Positive Control:LNCaP cell line
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes amino acids 100-104 of extracellular domain of human denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1). Species Crossreactivity: porcine, mouse and rat
Clone # USB：GCP-04
Isotype：IgG1
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Po Rt
Immunogen：Recombinant protein fragment corresponding to aa44-750 from human GCPII expressed in S2 cells
