83-5975-73　Fos (c-Fos) 100ul　046170

特徴

• The FOS gene and protein were originally identified as the transforming element in a viral oncogene.
• The transforming protein was named v-FOS, for viral FOS, and the normal cellular non-transforming proto-oncogene was called c-FOS, for cellular FOS.
• FOS is an acrynym for “FBJ murine osteogenic sarcoma”, the virus in which the gene product was first discovered.
• The c-FOS protein is a normal gene acting as an on/off switch controlling the expression of many other genes.
• The v-FOS form is mutated to stay in the on position, this persistently activating other genes and promoting unregulated cell division.
• The unmutated c-FOS is an “immediate-early” gene, so-called because protein expression is usually very low but increases rapidly and transiently in response to a wide array of stimuli including serum, growth factors, tumor promoters, cytokines, and UV radiation.
• Newly expressed c-FOS protein associates with JUN family and other basic leucine-zipper (bZIP) proteins to create a variety of activator protein-1 (AP-1) complexes (1).
• AP-1 complexes specifically activate the expression of many other genes and so regulate cellular responses to stimuli which may result in cell proliferation, differentiation, neoplastic transformation, apoptosis, and response to stress (2).
• The regulated expression of c-FOS therefore plays an important role in many cellular functions.
• Site specific phosphorylation activates c-FOS, while sumoylation of c-FOS inhibits the AP-1 transcriptional activity (3,4).
• Since c-FOS expression is induced in neurons which are rapidly firing action potentials, appropriate c-Fos antibodies can be used to identify activated neurons in tissues (5).
• Using current techniques it is possible to follow processes of such cells or obtain data on their mRNA expression.
• Can be used to identify activated cells in cell culture and in sections and to follow c-FOS expression in western blots of cell and tissue homogenates.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry/Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500Immunohistochemistry (formalin fixed paraffin embedded sections): 1:500Immunocytochemistry/Immunofluorescence: 1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.