Neuropilins are transmembrane proteins that contain two CUB domains (a1 and a2), two coagulation factor-like domains (b1 and b2), and a MAM domain in the extracellular region.
These proteins have short cytoplasmic domains that include a PDZ-binding motif.
The neuropilin (NRP) family includes NRP-1, NRP-2A, and NRP-2B.
NRP-1 has been implicated as a receptor involved in axon guidance and VEGF signaling.
NRP-1 mediates activation of intracellular signaling pathways through interaction with its co-receptors, Plexin-A1 and VEGFRs.
The expression of NRP-1, along with the co-receptor Plexin-A1, is upregulated in neurons after central nervous system injury.
The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury.
Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.
Applications:Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:250Immunohistochemistry: 1:100Immunocytochemistry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity：Recognizes human Neuropilin-1. Detects a 130kD protein corresponding to the apparent molecular mass of neuropilin-1 on SDS-PAGE immunoblots of COS-7 cells transfected with mouse neuropilin-1. In immunocytochemistry, this antibody co-localizes with anti-HA in Cos-7 cells transfected with HA-tagged mouse neuropilin-1. Species Crossreactivity: mouse and rat
Clone # USB：M534
Isotype：IgG1
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids within the a1 CUB domain of human neuropilin-1 (coupled to carrier protein). This sequence is highly conserved in rat and mouse neuropilin-1, and has low homology to neuropilin-2.
