83-5975-70　CART, aa28-116 (Cocaine And Amphetamine-Regulated Transcript) 500ug　046163

特徴

• CART, also known as cocaine and amphetamine-related transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations.
• In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment.
• There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region.
• There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist.
• These include a 5K, 48 aa peptide (CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89).
• Human CART is 98% aa identical to both moyuse and rat CART (1-89).
• Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions.
• In addition, anatomical location is associated with differing lengths of CART.
• For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/ml using recombinant human CARTImmunohistochemistry: 8-25ug/ml with perfusion fixed frozen sections of rat brain (cortex)Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.