Integrins are heterodimeric receptors comprised of an alpha and a beta subunit.
Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit.
Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin.
It is expressed on hepatoma cells, fibroblasts and carcinoma cells.
Applications:Suitable for use Flow Cytometry, Immunocytochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Immunocytochemistry: 8-25ug/ml in HT1080 human cell lineFlow Cytometry: 2.5ug/10e6 cells in MCF‑7 human breast cancer cell lineOptimal dilution determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20℃.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin: ≤0.10EU/ug (LAL)
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity：Recognizes human Integrin alpha V beta 5. Recognizes the human Integrin alpha V beta 5 heterodimer and does not recognize the alpha V subunit in association with any other beta subunits.
