83-5975-66　Adipophilin (ADFP, Adipose Differentiation-related Protein) 1mL　046150

特徴

• Adipophilin is a ubiquitous compound of lipid droplets.
• Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines and is inducible by etomoxir.
• Enhanced expression of ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation; such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma.
• It can also be a potent marker for atherosclerosis.
• It can also be used to study the virus entry of e.g.
• HCV via lipid droplets.
• Adipophilin is localized to glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver.
• Adipocytes do not display staining.
• Lipid-storing CD68-positive macrophages do positively stain for adipophilin.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (Frozen): 1:10-1:100 (0.5-5ug/ml)Immunohistochemistry (paraffin): 1:10-1:100 (0.5-5ug/ml microwave treatment recommended)Western Blot: 1:50-1:100 (0.5-1ug/ml)Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.