Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain.
Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex.
Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9.
Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins.
046145 recognizes cytochrome-c from most species and has been shown to be useful for Western blotting.
Applications:Suitable for use in Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 10ul/5mlImmunocytochemistry: 1-3ug/ml (1:150-1:500)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
抗体種類：Mab
宿主：mouse
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes equine cytochrome C. Species Crossreactivity: human, mouse, rat and hamster
