Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single-stranded RNA virus that belongs in a family of viruses known as coronaviruses.
SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal.
SARS-CoV-2 is structurally composed of 4 main proteins (e.g.
spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins.
The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells.
This protein is composed of two subunits, S1 and S2.
S1 is responsible for binding to the host cell via ACE2 receptor and the S2 subunit is responsible for fusion with the host cell.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.0125-0.1ug/mlELISA: 30.4ng/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4℃ for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20℃.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
抗体種類：Mab
宿主：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Specificity：Recognizes SARS-CoV-2 spike subunit 2 (S2) glycoprotein. This clone has been reported to show reactivity towards SARS-CoV (Ng OW, et al. 2014. PLoS One. 9:e102415; Lip KM, et al. 2006. J Virol. 80:941-950.)
Clone # USB：1A9/S2
Isotype：IgG1,k
Calc Applications Abbrev：E WB
Immunogen：Partial recombinant SARS-CoV protein corresponding to region of S2 subunit
