83-5975-54　Tau, aa157-168 (Tau Protein, Microtubule-associated protein tau, MAPT) 100ul　046134

特徴

• Tau proteins are microtubule-associated protein (MAPs) which are abundant in neurons of the central nervous system, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes and elsewhere.
• One of tau's main functions is to modulate the stability of axonal microtubules.
• Tau is active primarily in the distal portions of axons providing microtubule stabilization as well as flexibility.
• Pathologies and dementias of the nervous system such as Alzheimer's disease feature tau proteins that have become defective and no longer stabilize microtubules properly.
• As a result, tau forms aggregates with specific structural properties referred to as Paired Helical Filaments (PHFs) that are a characteristic of many different types of dementias, known as tauopathies.
• Tau has two primary ways of controlling microtubule stability: isoforms and phosphorylation.
• Six tau isoforms exist in human brain tissue, and they are distinguished by the number of binding domains.
• Three isoforms have three binding domains and the remaining three have four binding domains.
• The binding domains are located in the carboxy-terminus of the protein and are positively-charged (for binding to the negatively-charged microtubule).
• Tau isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/mlImmunohistochemistry (paraffin): 10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.