83-5975-47　GSK3B (Glycogen synthase kinase 3 beta, Serine/Threonine-Protein Kinase GSK3B, Glycogen Synthase Kinase-3 Beta) 100ug　046127

特徴

• GSK3B is a serine-threonine kinase and a member of the glycogen synthase kinase subfamily.
• It is a negative regulator of the hormonal control of glucose, Wnt signaling and also transcriptional factors and microtubules.
• It has been associated with regulation of several cellular mechanisms such as glycogen synthesis, protein synthesis, cell proliferation, microtubules stabilization, cell migration, autophagy and apoptosis.
• GSK3B is activated by phosphorylation at tyrosine 216 and inactivated by phosphorylation at serine 9.
• Defects on GSK3B has been associated with Alzheimer’s and Parkinson’s diseases.
• Applications:Suitable for use in Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry: 1.25-5ug/ml.
• 4% PFA fixation followed by permeabilization with ice-cold methanol, or fixation/permeabilization with ice cold methanol alone is recommended.
• We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X
• Immunohistochemistry (FFPE): 4-6ug/ml, antigen retrieval using citrate buffer, HIER, and overnight antibody incubation at 4℃
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.