83-5975-42　Nuclear Pore Complex Proteins 100ul　046122

特徴

• Nuclear pores are large protein complexes that cross the nuclear envelope.
• The proteins that make up the nuclear pore complex are known as nucleoporins.
• About half of the nucleoporins typically contain solenoid protein domains—either an alpha solenoid or a beta-propeller fold, or in some cases both as separate structural domains.
• Each NPC contains at least 456 individual protein molecules and is composed of 30 distinct proteins (nucleoporins).
• The other half show structural characteristics typical of "natively unfolded" or intrinsically disordered proteins, i.e.
• they are highly flexible proteins that lack ordered secondary structure.
• These disordered proteins are the FG nucleoporins, so called because their amino-acid sequence contains many phenylalanine—glycine repeats.
• 046122 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins.
• This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope.
• It is also useful in studying changes in the nuclear structure during mitosis and meiosis.
• Applications:Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry and Immunoelectron Microscopy.
• Other applications not tested.
• Recommended Dilutions:Immunocytochemistry (IF): 0.5-1ug/ml (1:1000-1:2000)Immunohistochemistry (formalin-fixed paraffin-embedded tissue): 0.5-1ug/mlImmunohistochemistry (frozen): 1:5000Western Blot: 0.2-0.5ug/ml (1:2000-1:5000)Immunoprecipitation: 1:5000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4℃ for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20℃.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.